A simple, selective and rapid Reversed Phase High Performance Liquid Chromatographic (RPHPLC) method for the analysis of Dasatinib in tablet has been developed and validated. The chromatographic system consisted of a Shimadzu LC-20 AT pump, SPD-20 A UV/Visible detector. The separation was achieved from a YMC Pack Pro C18 (150 x 4.6mm, 3�µm) at ambient temperature with a mobile phase containing 0.05M Ammonium acetate pH 5.25 buffer, Acetonitrile in the ratio of 67:33% v/v mix well and sonicate to degas for 5minutes. The samples were monitored at 320nm for detection at a flow rate of 1mL/min and the retention time was about 5.32minutes for Dasatinib. The method is selective and able to resolve drug peaks from formulation excipients. The calibration curve was linear over the concentration range of 100-600�µg/mL (R2 = 0.999). The proposed method is accurate in the range of 99.997% - 100.001% recovery and precise (%RSD of intraday variation were 0.0023-0.0041 and %RSD of inter day variation were 0.0007-0.0061). The method has been used to test market product (SPRYCEL) and potency was found within limit (99.991%- 100.013%). Therefore, this method can be used as a more convenient and efficient option for the analysis of Dasatinib in tablet dosage form.
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